CRKL monoclonal antibody, clone 5E12
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis using CRKL monoclonal antibody, clone 5E12 (Cat # MAB10613) against human CRKL (aa 100-303) recombinant protein. (Expected MW is 47.8 kDa)
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of CRKL monoclonal antibody, clone 5E12 (Cat # MAB10613) .
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using CRKL monoclonal antibody, clone 5E12 (Cat # MAB10613) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CRKL.
Immunogen
Recombinant protein corresponding to human CRKL.
Host
Mouse
Theoretical MW (kDa)
39
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using CRKL monoclonal antibody, clone 5E12 (Cat # MAB10613) against human CRKL (aa 100-303) recombinant protein. (Expected MW is 47.8 kDa)Enzyme-linked Immunoabsorbent Assay
ELISA measurement of CRKL monoclonal antibody, clone 5E12 (Cat # MAB10613) .Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using CRKL monoclonal antibody, clone 5E12 (Cat # MAB10613) (blue) and negative control (red). -
Gene Info — CRKL
Entrez GeneID
1399Gene Name
CRKL
Gene Alias
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Gene Description
v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Omim ID
602007Gene Ontology
HyperlinkGene Summary
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic
Other Designations
v-crk avian sarcoma virus CT10 oncogene homolog-like
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Interactome
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Pathway
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Disease
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