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CREB1 monoclonal antibody, clone 5G3

  • Catalog # : MAB10612
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CREB1.
  • Immunogen:
  • Recombinant protein corresponding to human CREB1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 43
  • Reactivity:
  • Human, Monkey, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) against K-562 (1) , Jurkat (2) , L1210 (3) , HEK293 (4) , A-431 (5) , HeLa (6) , COS-7 (7) , PC-12 (8) , and NIH/3T3 (9) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues (upper) and submaxillary tumor tissues (bottom) using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA measurement of CREB1 monoclonal antibody, clone 5G3 (Cat # MAB10612) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1385
  • Gene Name:
  • CREB1
  • Gene Alias:
  • CREB,MGC9284
  • Gene Description:
  • cAMP responsive element binding protein 1
  • Gene Summary:
  • This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • active transcription factor CREB,cAMP-response element-binding protein-1,transactivator protein
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