COX4I1 monoclonal antibody, clone 6B3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using COX4I1 monoclonal antibody, clone 6B3 (Cat # MAB10611) against HEK293 (1) , A-549 (2) and PC-12 (3) cell lysate.
Immunofluorescence
Immunofluorescence analysis of PANC-1 cells using COX4I1 monoclonal antibody, clone 6B3 (Cat # MAB10611) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of K-562 cells using COX4I1 monoclonal antibody, clone 6B3 (Cat # MAB10611) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant COX4I1.
Immunogen
Recombinant protein corresponding to human COX4I1.
Host
Mouse
Theoretical MW (kDa)
19
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using COX4I1 monoclonal antibody, clone 6B3 (Cat # MAB10611) against HEK293 (1) , A-549 (2) and PC-12 (3) cell lysate.Immunofluorescence
Immunofluorescence analysis of PANC-1 cells using COX4I1 monoclonal antibody, clone 6B3 (Cat # MAB10611) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of K-562 cells using COX4I1 monoclonal antibody, clone 6B3 (Cat # MAB10611) (blue) and negative control (red). -
Gene Info — COX4I1
Entrez GeneID
1327Gene Name
COX4I1
Gene Alias
COX4, COXIV, MGC72016
Gene Description
cytochrome c oxidase subunit IV isoform 1
Omim ID
123864Gene Ontology
HyperlinkGene Summary
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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