BIRC5 monoclonal antibody, clone 1H5
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against HEK293 (1) and BIRC5 (aa 1-142) -hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against human BIRC5 (aa 1-142) recombinant protein. (Expected MW is 16 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (upper) and human colon cancer (bottom) tissues using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of MSCs cells using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) .
Flow Cytometry
Flow cytometric analysis of Jurkat cells using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BIRC5.
Immunogen
Recombinant protein corresponding to human BIRC5.
Host
Mouse
Theoretical MW (kDa)
16
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against HEK293 (1) and BIRC5 (aa 1-142) -hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against human BIRC5 (aa 1-142) recombinant protein. (Expected MW is 16 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer (upper) and human colon cancer (bottom) tissues using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) with DAB staining.Immunofluorescence
Immunofluorescence analysis of MSCs cells using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) .Flow Cytometry
Flow cytometric analysis of Jurkat cells using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) (green) and negative control (red). -
Gene Info — BIRC5
Entrez GeneID
332Gene Name
BIRC5
Gene Alias
API4, EPR-1
Gene Description
baculoviral IAP repeat-containing 5
Omim ID
603352Gene Ontology
HyperlinkGene Summary
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq
Other Designations
apoptosis inhibitor 4|baculoviral IAP repeat-containing protein 5|survivin variant 3 alpha
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Interactome
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Pathway
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Disease
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