GATA1 monoclonal antibody, clone 4F5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using GATA1 monoclonal antibody, clone 4F5 (Cat # MAB10607) against K-562 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer, using GATA1 monoclonal antibody, clone 4F5 (Cat # MAB10607) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of K-562 cells using GATA1 monoclonal antibody, clone 4F5 (Cat # MAB10607) (green) . Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant GATA1.
Immunogen
Recombinant protein corresponding to human GATA1.
Host
Mouse
Theoretical MW (kDa)
42.7
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using GATA1 monoclonal antibody, clone 4F5 (Cat # MAB10607) against K-562 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer, using GATA1 monoclonal antibody, clone 4F5 (Cat # MAB10607) with DAB staining.Immunofluorescence
Immunofluorescence analysis of K-562 cells using GATA1 monoclonal antibody, clone 4F5 (Cat # MAB10607) (green) . Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GATA1
Entrez GeneID
2623Gene Name
GATA1
Gene Alias
ERYF1, GF-1, GF1, NFE1
Gene Description
GATA binding protein 1 (globin transcription factor 1)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq
Other Designations
GATA binding protein 1|NF-E1 DNA-binding protein|OTTHUMP00000023259|erythroid transcription factor 1|globin transcription factor 1|transcription factor GATA1
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Interactome
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Disease
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