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KRT8 monoclonal antibody, clone 8A5D12

  • Catalog # : MAB10601
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant KRT8.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 391-483 of human KRT8.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 54
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) against A-549 (1) , HeLa (2) , MCF-7 (3) , A-431 (4) and HepG2 (5) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissues using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) , lung cancer (B) and ovarian cancer tissue (C) , showing membrane and cytoplasmic localization with DAB staining using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (upper) and HepG2 cells (bottom) using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3856
  • Gene Name:
  • KRT8
  • Gene Alias:
  • CARD2,CK8,CYK8,K2C8,K8,KO
  • Gene Description:
  • keratin 8
  • Gene Summary:
  • This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq
  • Other Designations:
  • cytokeratin 8,keratin, type II cytoskeletal 8
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