Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) , lung cancer (B) and ovarian cancer tissue (C) , showing membrane and cytoplasmic localization with DAB staining using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) .
Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (upper) and HepG2 cells (bottom) using KRT8 monoclonal antibody, clone 8A5D12 (Cat # MAB10601) (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq