KIR2DS4 monoclonal antibody, clone 5F2
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More Files
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Western Blot (Recombinant protein)
Western blot analysis of recombinant human KIR2DL1 , KIR2DL3 , KIR2DL4 and KIR2DS4 (each 100 ng) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with KIR2DS4 monoclonal antibody , clone 5F2 (1 : 1000) (Cat # MAB1060) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates recombinant human KIR2DS4 protein.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KIR2DS4.
Immunogen
Recombinant protein corresponding to amino acids 23-223 of human KIR2DS4.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G affinity chromatography
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of recombinant human KIR2DL1 , KIR2DL3 , KIR2DL4 and KIR2DS4 (each 100 ng) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with KIR2DS4 monoclonal antibody , clone 5F2 (1 : 1000) (Cat # MAB1060) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates recombinant human KIR2DS4 protein.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KIR2DS4
Entrez GeneID
3809Protein Accession#
P43632Gene Name
KIR2DS4
Gene Alias
CD158I, KIR1D, KIR412, KKA3, MGC120019, MGC125315, MGC125317, NKAT8
Gene Description
killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4
Omim ID
604955Gene Ontology
HyperlinkGene Summary
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq
Other Designations
KIR antigen 2DS4|killer inhibitory receptor 4-1-2|natural killer cell inhibitory receptor
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Pathway
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Disease
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Publication Reference
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Nucleotide and amino acid sequence alignment for human killer cell inhibitory receptors (KIR), 1998.
Steffens U, Vyas Y, Dupont B, Selvakumar A.
Tissue Antigens 1998 Apr; 51(4 Pt 1):398.
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Molecular basis of HLA-C recognition by p58 natural killer cell inhibitory receptors.
Kim J, Chwae YJ, Kim MY, Choi IH, Park JH, Kim SJ.
Journal of Immunology 1997 Oct; 159(8):3875.
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Molecular clones of the p58 NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains.
Wagtmann N, Biassoni R, Cantoni C, Verdiani S, Malnati MS, Vitale M, Bottino C, Moretta L, Moretta A, Long EO.
Immunity 1995 May; 2(5):439.
Application：Flow Cyt, Human, Human B cells.
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Nucleotide and amino acid sequence alignment for human killer cell inhibitory receptors (KIR), 1998.
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