EPHB1 monoclonal antibody, clone 5F10A4
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EPHB1.
Immunogen
Recombinant protein corresponding to amino acids 19-133 of human EPHB1.
Host
Mouse
Theoretical MW (kDa)
110
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using EPHB1 monoclonal antibody, clone 5F10A4 (Cat # MAB10595) against MDA-MB-468 (1) , MDA-MB-453 (2) , MCF-7 (3) , T-47D (4) and SK-BR-3 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and colon cancer (bottom) showing cytoplasmic localization with DAB staining using EPHB1 monoclonal antibody, clone 5F10A4 (Cat # MAB10595) .Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPHB1
Entrez GeneID
2047Gene Name
EPHB1
Gene Alias
ELK, EPHT2, FLJ37986, Hek6, NET
Gene Description
EPH receptor B1
Omim ID
600600Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
eph tyrosine kinase 2|ephrin receptor EphB1|soluble EPHB1 variant 1
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Interactome
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