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EPHB2 monoclonal antibody, clone 2D12C6

  • Catalog # : MAB10592
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant EPHB2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 17-200 of human EPHB2.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis using EPHB2 monoclonal antibody, clone 2D12C6 (Cat # MAB10592) against truncated EPHB2 recombinant protein (1) and extracellular EPHB2 (aa 19-476) -hIgGFc transfected CHO-K1 cell lysate (2) .
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using EPHB2 monoclonal antibody, clone 2D12C6 (Cat # MAB10592) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2048
  • Gene Name:
  • EPHB2
  • Gene Alias:
  • CAPB,DRT,EPHT3,ERK,Hek5,MGC87492,PCBC,Tyro5
  • Gene Description:
  • EPH receptor B2
  • Gene Summary:
  • Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000002914,OTTHUMP00000002916,developmentally-regulated eph-related tyrosine kinase,elk-related tyrosine kinase,eph tyrosine kinase 3,ephrin receptor EphB2,prostate cancer-brain cancer susceptibility
  • Gene Pathway
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