EPHB2 monoclonal antibody, clone 2D12C6
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More Files
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Western Blot
Western blot analysis using EPHB2 monoclonal antibody, clone 2D12C6 (Cat # MAB10592) against truncated EPHB2 recombinant protein (1) and extracellular EPHB2 (aa 19-476) -hIgGFc transfected CHO-K1 cell lysate (2) .
Immunofluorescence
Immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using EPHB2 monoclonal antibody, clone 2D12C6 (Cat # MAB10592) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant EPHB2.
Immunogen
Recombinant protein corresponding to amino acids 17-200 of human EPHB2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using EPHB2 monoclonal antibody, clone 2D12C6 (Cat # MAB10592) against truncated EPHB2 recombinant protein (1) and extracellular EPHB2 (aa 19-476) -hIgGFc transfected CHO-K1 cell lysate (2) .Immunofluorescence
Immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using EPHB2 monoclonal antibody, clone 2D12C6 (Cat # MAB10592) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPHB2
Entrez GeneID
2048Gene Name
EPHB2
Gene Alias
CAPB, DRT, EPHT3, ERK, Hek5, MGC87492, PCBC, Tyro5
Gene Description
EPH receptor B2
Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
OTTHUMP00000002914|OTTHUMP00000002916|developmentally-regulated eph-related tyrosine kinase|elk-related tyrosine kinase|eph tyrosine kinase 3|ephrin receptor EphB2|prostate cancer-brain cancer susceptibility
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Interactome
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Pathway
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Disease
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