Western blot analysis human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS - PAGE, transferred to PVDF membrane and probed with KIR2DL4 monoclonal antibody, clone 2H6 (1 : 500) (Cat # MAB1059). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system. Lane 1 : Extracellular domain of KIR2DL1 (23 - 223 aa). Lane 2 : Extracellular domain of KIR2DL3 (23 - 223 aa). Lane 3 : Extracellular domain of KIR2DL4 (44 - 202 aa).
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternative splicing results in multiple transcript variants. [provided by RefSeq
NK cell receptor,OTTHUMP00000068610,natural killer cell inhibitory receptor