KIR2DL4 monoclonal antibody, clone 2H6
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More Files
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Western Blot (Recombinant protein)
Western blot analysis human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS - PAGE, transferred to PVDF membrane and probed with KIR2DL4 monoclonal antibody, clone 2H6 (1 : 500) (Cat # MAB1059). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system. Lane 1 : Extracellular domain of KIR2DL1 (23 - 223 aa). Lane 2 : Extracellular domain of KIR2DL3 (23 - 223 aa). Lane 3 : Extracellular domain of KIR2DL4 (44 - 202 aa).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KIR2DL4.
Immunogen
Recombinant protein corresponding to amino acids 44-202 of human KIR2DL4.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes KIR2DL1, KIR2DL3, KIR2DL4 and KIR2DS4, members of killer cell immunoglobulin-like receptor family.
Form
Liquid
Purification
Protein G affinity chromatography
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis human recombinant protein KIR2DL1, KIR2DL3 and KIR2DL4 (each 20 ng per well) were resolved by SDS - PAGE, transferred to PVDF membrane and probed with KIR2DL4 monoclonal antibody, clone 2H6 (1 : 500) (Cat # MAB1059). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system. Lane 1 : Extracellular domain of KIR2DL1 (23 - 223 aa). Lane 2 : Extracellular domain of KIR2DL3 (23 - 223 aa). Lane 3 : Extracellular domain of KIR2DL4 (44 - 202 aa).Enzyme-linked Immunoabsorbent Assay
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Gene Info — KIR2DL4
Entrez GeneID
3805GeneBank Accession#
NM_002255Protein Accession#
Q99706Gene Name
KIR2DL4
Gene Alias
CD158D, G9P, KIR103, KIR103AS
Gene Description
killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4
Omim ID
604945Gene Ontology
HyperlinkGene Summary
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the "framework" loci that is present on all haplotypes. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
NK cell receptor|OTTHUMP00000068610|natural killer cell inhibitory receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Molecular mechanism of the activation-induced cell death inhibition mediated by a p70 inhibitory killer cell Ig-like receptor in Jurkat T cells.
Chwae YJ, Chang MJ, Park SM, Yoon H, Park HJ, Kim SJ, Kim J.
The Journal of Immunology 2002 Oct; 169(7):3726.
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Monoclonal antibodies with various reactivity to p58 killer inhibitory receptors.
Shin JS, Shin EC, Kim J, Choi IH, Park JH, Kim SJ.
Hybridoma 1999 Dec; 18(6):521.
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Molecular mechanism of the activation-induced cell death inhibition mediated by a p70 inhibitory killer cell Ig-like receptor in Jurkat T cells.
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