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Last updated: 2022/7/3

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MKI67 monoclonal antibody, clone 8D5 

  • Catalog # : MAB10582
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of MKI67.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to human MKI67.
  • Sequence:
  • CEDLAGFKELFQTPG
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 358
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using MKI67 monoclonal antibody, clone 8D5 (Cat # MAB10582) against HeLa (1) , MCF-7 (2) and Raji (3) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and rectal cancer (bottom) using MKI67 monoclonal antibody, clone 8D5 (Cat # MAB10582) with DAB staining.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 4288
  • Gene Name:
  • MKI67
  • Gene Alias:
  • KIA,Ki-67
  • Gene Description:
  • antigen identified by monoclonal antibody Ki-67
  • Gene Summary:
  • This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020739,proliferation-related Ki-67 antigen
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