Western blot analysis of human recombinant proteins, extracellular domain of NCR3 (50 ng) was resolved by SDS - PAGE, transferred to PVDF membrane and probed with NCR3 monoclonal antibody, clone y4E6 (1 : 1000) (Cat # MAB1058). Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates human recombinant protein NCR3 (residues 19 ~ 138).
Natural cytotoxicity receptors (NCRs), such as NCR3, are activating natural killer (NK) cell receptors that belong to the immunoglobulin (Ig) superfamily. NCR3 is expressed in all resting and activated NK cells and forms a complex with CD3-zeta (CD3Z, or CD247; MIM 186780) (Sato et al., 2001 [PubMed 11782277]).[supplied by OMIM