COTL1 monoclonal antibody, clone 5C8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571) against HEK293 (1) and COTL1 (aa : 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571) against human COTL1 (aa : 1-142) recombinant protein. (Expected MW is 16 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (A) and human liver cancer (B) tissues using COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571).
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant COTL1.
Immunogen
Recombinant protein corresponding to human COTL1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571) against HEK293 (1) and COTL1 (aa : 1-142)-hIgGFc transfected HEK293 (2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571) against human COTL1 (aa : 1-142) recombinant protein. (Expected MW is 16 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer (A) and human liver cancer (B) tissues using COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA detection with COTL1 monoclonal antibody, clone 5C8 (Cat # MAB10571). -
Gene Info — COTL1
Entrez GeneID
23406Gene Name
COTL1
Gene Alias
CLP, FLJ43657, MGC19733
Gene Description
coactosin-like 1 (Dictyostelium)
Omim ID
606748Gene Ontology
HyperlinkGene Summary
This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq
Other Designations
coactosin-like 1
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Interactomes
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Diseases
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