ADRBK1 monoclonal antibody, clone 3F8
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569) against HeLa (1), Jurkat (2), MOLT 4 (3), Raji (4), THP-1 (5), L1210 (6), COS-7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues (A) and cervical cancer tissues (B) using ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569) (green). Blue: DRAQ5 fluorescent DNA dye.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ADRBK1.
Immunogen
Recombinant protein corresponding to human ADRBK1.
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569) against HeLa (1), Jurkat (2), MOLT 4 (3), Raji (4), THP-1 (5), L1210 (6), COS-7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues (A) and cervical cancer tissues (B) using ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569) with DAB staining.Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
ELISA detection with ADRBK1 monoclonal antibody, clone 3F8 (Cat # MAB10569). -
Gene Info — ADRBK1
Entrez GeneID
156Gene Name
ADRBK1
Gene Alias
BARK1, BETA-ARK1, FLJ16718, GRK2
Gene Description
adrenergic, beta, receptor kinase 1
Omim ID
109635Gene Ontology
HyperlinkGene Summary
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq
Other Designations
beta adrenergic receptor kinase 1
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Interactome
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Pathway
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Disease
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