LIN28 monoclonal antibody, clone 6D1F9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using LIN28 monoclonal antibody, clone 6D1F9 (Cat # MAB10567) against NTERA-2 cell lysate.
Immunofluorescence
Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 monoclonal antibody, clone 6D1F9 (Cat # MAB10567) (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of methanol fixed HeLa cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant LIN28.
Immunogen
Recombinant protein corresponding to amino acids 93-209 of human LIN28.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.4 (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using LIN28 monoclonal antibody, clone 6D1F9 (Cat # MAB10567) against NTERA-2 cell lysate.Immunohistochemistry
Immunofluorescence
Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 monoclonal antibody, clone 6D1F9 (Cat # MAB10567) (green). Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal immunofluorescence analysis of methanol fixed HeLa cells were transfected with pMX construct of human LIN28, cells were analyzed ~62 hours after transfection.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LIN28
Entrez GeneID
79727Gene Name
LIN28
Gene Alias
CSDD1, FLJ12457, LIN-28, LIN28A, ZCCHC1
Gene Description
lin-28 homolog (C. elegans)
Omim ID
611043Gene Ontology
HyperlinkGene Summary
CCHC domain containing 1
Other Designations
OTTHUMP00000003663|RNA-binding protein LIN-28|lin-28 homolog|zinc finger, CCHC domain containing 1
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