RASSF1 monoclonal antibody, clone 3F3
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cells.
Immunocytochemistry
Immunocytochemistry of A431 cells. The cell was stained with RASSF1 monoclonal antibody, clone 3F3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue)
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant RASSF1.
Immunogen
Recombinant protein corresponding to amino acids 1-340 of human RASSF1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A affinity chromatography
Isotype
IgG1, kappa
Recommend Usage
ELISA
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cells.Immunocytochemistry
Immunocytochemistry of A431 cells. The cell was stained with RASSF1 monoclonal antibody, clone 3F3 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue)Enzyme-linked Immunoabsorbent Assay
-
Gene Info — RASSF1
Entrez GeneID
11186Protein Accession#
Q9NS23Gene Name
RASSF1
Gene Alias
123F2, NORE2A, RASSF1A, RDA32, REH3P21
Gene Description
Ras association (RalGDS/AF-6) domain family member 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000195503|Ras association (RalGDS/AF-6) domain family 1|Ras association domain family 1|Ras association domain family protein 1|WUGSC:H_LUCA12.5|cardiac-specific ras association domain family 1 protein|pancreas-specific ras association domain fam
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Hypermethylation of RASSF1A in human and rhesus placentas.
Chiu RW, Chim SS, Wong IH, Wong CS, Lee WS, To KF, Tong JH, Yuen RK, Shum AS, Chan JK, Chan LY, Yuen JW, Tong YK, Weier JF, Ferlatte C, Leung TN, Lau TK, Lo KW, Lo YM.
The American Journal of Pathology 2007 Mar; 170(3):941.
Application：IHC-P, Human, first trimester placental tissues.
-
Chromatin inactivation precedes de novo DNA methylation during the progressive epigenetic silencing of the RASSF1A promoter.
Strunnikova M, Schagdarsurengin U, Kehlen A, Garbe JC, Stampfer MR, Dammann R.
Molecular and Cellular Biology 2005 May; 25(10):3923.
-
The tumor suppressor RASSF1A in human carcinogenesis: an update.
Dammann R, Schagdarsurengin U, Seidel C, Strunnikova M, Rastetter M, Baier K, Pfeifer GP.
Histology and Histopathology 2005 Apr; 20(2):645.
Application：IHC, WB, Human, Human cancers, Mammalian cells.
-
Hypermethylation of RASSF1A in human and rhesus placentas.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com