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Last updated: 2017/2/19
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RASSF1 monoclonal antibody, clone 3F3

  • Catalog # : MAB1056
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant RASSF1.
  • Immunogen:
  • Recombinant protein corresponding to full length human RASSF1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HEK 293 (1) , A - 549 (2) and HeLa (3) cells extracts were resolved by SDS - PAGE , transferred to PVDF membrane and probed with RASSF1 monoclonal antibody , clone 3F3 (1 : 1000) (Cat # MAB1056) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemistry of A-431 cells were labeled with RASSF1 monoclonal antibody , clone 3F3 (Cat # MAB1056) , and detection was using a biotinylated secondary antibody and Texas - red conj streptavidin.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Gene Name:
  • RASSF1
  • Gene Alias:
  • 123F2,NORE2A,RASSF1A,RDA32,REH3P21
  • Gene Description:
  • Ras association (RalGDS/AF-6) domain family member 1
  • Gene Summary:
  • This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000195503,Ras association (RalGDS/AF-6) domain family 1,Ras association domain family 1,Ras association domain family protein 1,WUGSC:H_LUCA12.5,cardiac-specific ras association domain family 1 protein,pancreas-specific ras association domain fam
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