Western blot analysis of HEK 293 (1) , A - 549 (2) and HeLa (3) cells extracts were resolved by SDS - PAGE , transferred to PVDF membrane and probed with RASSF1 monoclonal antibody , clone 3F3 (1 : 1000) (Cat # MAB1056) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a ECL detection system.
Immunocytochemistry of A-431 cells were labeled with RASSF1 monoclonal antibody , clone 3F3 (Cat # MAB1056) , and detection was using a biotinylated secondary antibody and Texas - red conj streptavidin.
This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
OTTHUMP00000195503,Ras association (RalGDS/AF-6) domain family 1,Ras association domain family 1,Ras association domain family protein 1,WUGSC:H_LUCA12.5,cardiac-specific ras association domain family 1 protein,pancreas-specific ras association domain fam