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Last updated: 2017/2/19
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CHD3 monoclonal antibody, clone 2G4

  • Catalog # : MAB10553
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CHD3.
  • Immunogen:
  • Recombinant protein corresponding to human CHD3.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 226
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) against HeLa (1), K-562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) (green). Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K-562 cells using CHD3 monoclonal antibody, clone 2G4 (Cat # MAB10553) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1107
  • Gene Name:
  • CHD3
  • Gene Alias:
  • Mi-2a,Mi2-ALPHA,ZFH
  • Gene Description:
  • chromodomain helicase DNA binding protein 3
  • Gene Summary:
  • This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • zinc-finger helicase (Snf2-like)
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