CDK5 monoclonal antibody, clone 4E4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) against HeLa (1), K-562 (2), PC-12 (3) and COS-7 (4) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissues (A) and brain tissues (B) using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of GC7901 cells using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549).
Flow Cytometry
Flow cytometric analysis of K-562 cells using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CDK5.
Immunogen
Recombinant protein corresponding to human CDK5.
Host
Mouse
Theoretical MW (kDa)
35
Reactivity
Human, Monkey, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) against HeLa (1), K-562 (2), PC-12 (3) and COS-7 (4) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissues (A) and brain tissues (B) using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) with DAB staining.Immunofluorescence
Immunofluorescence analysis of GC7901 cells using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549).Flow Cytometry
Flow cytometric analysis of K-562 cells using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) (green) and negative control (purple). -
Gene Info — CDK5
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Interactome
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Pathway
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Disease
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