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Last updated: 2016/12/4
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CDK5 monoclonal antibody, clone 4E4

  • Catalog # : MAB10549
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CDK5.
  • Immunogen:
  • Recombinant protein corresponding to human CDK5.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 35
  • Reactivity:
  • Human, Monkey, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) against HeLa (1), K-562 (2), PC-12 (3) and COS-7 (4) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissues (A) and brain tissues (B) using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of GC7901 cells using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA detection with CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K-562 cells using CDK5 monoclonal antibody, clone 4E4 (Cat # MAB10549) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1020
  • Gene Name:
  • CDK5
  • Gene Alias:
  • PSSALRE
  • Gene Description:
  • cyclin-dependent kinase 5
  • Other Designations:
  • protein kinase CDK5 splicing
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