CDC27 monoclonal antibody, clone 5C12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CDC27 monoclonal antibody, clone 5C12 (Cat # MAB10545) against CDC27-hIgGFc transfected HEK293 cell.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (A) and colon cancer tissues (B) using CDC27 monoclonal antibody, clone 5C12 (Cat # MAB10545) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CDC27.
Immunogen
Recombinant protein corresponding to human CDC27.
Host
Mouse
Theoretical MW (kDa)
91
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CDC27 monoclonal antibody, clone 5C12 (Cat # MAB10545) against CDC27-hIgGFc transfected HEK293 cell.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (A) and colon cancer tissues (B) using CDC27 monoclonal antibody, clone 5C12 (Cat # MAB10545) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC27
Entrez GeneID
996Gene Name
CDC27
Gene Alias
APC3, CDC27Hs, D0S1430E, D17S978E, HNUC
Gene Description
cell division cycle 27 homolog (S. cerevisiae)
Omim ID
116946Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
anaphase-promoting complex, protein 3|cell division cycle protein 27|nuc2 homolog
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Interactome
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Pathway
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