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CDC27 monoclonal antibody, clone 5C12

  • Catalog # : MAB10545
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CDC27.
  • Immunogen:
  • Recombinant protein corresponding to human CDC27.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 91
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using CDC27 monoclonal antibody, clone 5C12 (Cat # MAB10545) against CDC27-hIgGFc transfected HEK293 cell.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissues (A) and colon cancer tissues (B) using CDC27 monoclonal antibody, clone 5C12 (Cat # MAB10545) with DAB staining.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 996
  • Gene Name:
  • CDC27
  • Gene Alias:
  • APC3,CDC27Hs,D0S1430E,D17S978E,HNUC
  • Gene Description:
  • cell division cycle 27 homolog (S. cerevisiae)
  • Gene Summary:
  • The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • anaphase-promoting complex, protein 3,cell division cycle protein 27,nuc2 homolog
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