CD276 monoclonal antibody, clone 6A1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A) and ovarian cancer tissues (B) using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) with DAB staining.
Flow Cytometry
Flow cytometric analysis of PC-3 cells using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CD276.
Immunogen
Recombinant protein corresponding to human CD276.
Host
Mouse
Theoretical MW (kDa)
57
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A) and ovarian cancer tissues (B) using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PC-3 cells using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) (green) and negative control (purple). -
Gene Info — CD276
Entrez GeneID
80381Gene Name
CD276
Gene Alias
B7-H3, B7H3
Gene Description
CD276 molecule
Omim ID
605715Gene Ontology
HyperlinkGene Summary
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM
Other Designations
B7 homolog 3|CD276 antigen
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Interactome
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Pathway
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