Mouse monoclonal antibody raised against partial recombinant CD276.
Immunogen:
Recombinant protein corresponding to human CD276.
Host:
Mouse
Theoretical MW (kDa):
57
Reactivity:
Human
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000) Western Blot (1:500-1:2000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A) and ovarian cancer tissues (B) using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of PC-3 cells using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) (green) and negative control (purple).
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM