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Last updated: 2022/8/14
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CD276 monoclonal antibody, clone 6A1 

  • Catalog # : MAB10532
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CD276.
  • Immunogen:
  • Recombinant protein corresponding to human CD276.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 57
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) against HEK293 (1) and CD276-hIgGFc transfected HEK293 (2) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (A) and ovarian cancer tissues (B) using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) with DAB staining.
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of PC-3 cells using CD276 monoclonal antibody, clone 6A1 (Cat # MAB10532) (green) and negative control (purple).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • CD276
  • Gene Alias:
  • B7-H3,B7H3
  • Gene Description:
  • CD276 molecule
  • Gene Summary:
  • Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM
  • Other Designations:
  • B7 homolog 3,CD276 antigen
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