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Last updated: 2017/2/26
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CD247 monoclonal antibody, clone 4B10

  • Catalog # : MAB10531
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CD247.
  • Immunogen:
  • Recombinant protein corresponding to human CD247.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 18
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) against CD247-hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human thymus tissues using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of K-562 cells using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) (green). Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of Jurkat cells using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) (green) and negative control (purple).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 919
  • Gene Name:
  • CD247
  • Gene Alias:
  • CD3-ZETA,CD3H,CD3Q,CD3Z,T3Z,TCRZ
  • Gene Description:
  • CD247 molecule
  • Gene Summary:
  • The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CD247 antigen, zeta subunit,CD3Z antigen, zeta polypeptide (TiT3 complex),OTTHUMP00000032544,T-cell antigen receptor complex, zeta subunit of CD3,T-cell receptor T3 zeta chain,T-cell receptor zeta chain,T-cell surface glycoprotein CD3 zeta chain
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