CD247 monoclonal antibody, clone 4B10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) against CD247-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thymus tissues using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531).
Immunofluorescence
Immunofluorescence analysis of K-562 cells using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CD247.
Immunogen
Recombinant protein corresponding to human CD247.
Host
Mouse
Theoretical MW (kDa)
18
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) against CD247-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thymus tissues using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531).Immunofluorescence
Immunofluorescence analysis of K-562 cells using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD247 monoclonal antibody, clone 4B10 (Cat # MAB10531) (green) and negative control (purple). -
Gene Info — CD247
Entrez GeneID
919Gene Name
CD247
Gene Alias
CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ
Gene Description
CD247 molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD247 antigen, zeta subunit|CD3Z antigen, zeta polypeptide (TiT3 complex)|OTTHUMP00000032544|T-cell antigen receptor complex, zeta subunit of CD3|T-cell receptor T3 zeta chain|T-cell receptor zeta chain|T-cell surface glycoprotein CD3 zeta chain
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Interactome
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Pathway
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Disease
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