SETD7 monoclonal antibody, clone s4E5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysate.
Immunocytochemistry
Immunocytochemistry analysis of HeLa cells. The cell was stained with SETD7 monoclonal antibody, clone s4E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of Jurkat cell line, staining at 2-5 ug for 1x106(red line) cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant SETD7.
Immunogen
Recombinant protein corresponding to amino acids 1-366 of human SETD7.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A affinity chromatography
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T cell lysate.Immunocytochemistry
Immunocytochemistry analysis of HeLa cells. The cell was stained with SETD7 monoclonal antibody, clone s4E5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of Jurkat cell line, staining at 2-5 ug for 1x106(red line) cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — SETD7
Entrez GeneID
80854GeneBank Accession#
NM_030648Protein Accession#
Q8WTS6Gene Name
SETD7
Gene Alias
FLJ21193, KIAA1717, KMT7, SET7, SET7/9, SET9
Gene Description
SET domain containing (lysine methyltransferase) 7
Omim ID
606594Gene Ontology
HyperlinkOther Designations
H3-K4-HMTase|SET domain-containing protein 7|histone H3-K4 methyltransferase|histone H3-lysine 4-specific methyltransferase
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Interactome
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Pathway
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Disease
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Publication Reference
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Protein demethylation required for DNA methylation.
Hotz HR, Peters AH.
Nature Genetics 2009 Jan; 41(1):10.
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Structure and catalytic mechanism of the human histone methyltransferase SET7/9.
Xiao B, Jing C, Wilson JR, Walker PA, Vasisht N, Kelly G, Howell S, Taylor IA, Blackburn GM, Gamblin SJ.
Nature 2003 Jan; 421(6923):652.
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Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet.
Kwon T, Chang JH, Kwak E, Lee CW, Joachimiak A, Kim YC, Lee J, Cho Y.
The EMBO Journal 2003 Jan; 22(2):292.
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Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.
Nishioka K, Chuikov S, Sarma K, Erdjument-Bromage H, Allis CD, Tempst P, Reinberg D.
Genes & Development 2002 Feb; 16(4):479.
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Protein demethylation required for DNA methylation.
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