PROM1 monoclonal antibody, clone 3F10
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and human esophageal cancer tissues (B) using PROM1 monoclonal antibody, clone 3F10 (Cat # MAB10525) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with PROM1 monoclonal antibody, clone 3F10 (Cat # MAB10525).
Flow Cytometry
Flow cytometric analysis of HeLa cells using PROM1 monoclonal antibody, clone 3F10 (Cat # MAB10525) (blue) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of PROM1.
Immunogen
A synthetic peptide corresponding to amino acids 20-36 of human PROM1.
Sequence
GGQPSSTDAPKAWNYEL
Host
Mouse
Theoretical MW (kDa)
133
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and human esophageal cancer tissues (B) using PROM1 monoclonal antibody, clone 3F10 (Cat # MAB10525) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA detection with PROM1 monoclonal antibody, clone 3F10 (Cat # MAB10525).Flow Cytometry
Flow cytometric analysis of HeLa cells using PROM1 monoclonal antibody, clone 3F10 (Cat # MAB10525) (blue) and negative control (red). -
Gene Info — PROM1
Entrez GeneID
8842Gene Name
PROM1
Gene Alias
AC133, CD133, MSTP061, PROML1, RP41
Gene Description
prominin 1
Omim ID
604365Gene Ontology
HyperlinkGene Summary
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
hProminin|hematopoietic stem cell antigen|prominin-like 1
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Interactome
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Disease
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Publication Reference
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All-trans retinoic acid suppresses malignant characteristics of CD133-positive thyroid cancer stem cells and induces apoptosis.
Mei D, Lv B, Chen B, Xiao S, Jiang J, Xie Y, Jiang L.
PLoS One 2017 Aug; 12(8):e0182835.
Application：IF, IHC-P, Human, ARO, TT2609, and BHP10-3 cell lines, Human thyroid tumor tissues.
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Evaluation of Breast Cancer Stem Cells and Intratumor Stemness Heterogeneity in Triple-negative Breast Cancer as Prognostic Factors.
Yang F, Cao L, Sun Z, Jin J, Fang H, Zhang W, Guan X.
International Journal of Biological Sciences 2016 Dec; 12(12):1568.
Application：IHC-P, Human, Human breast cancer.
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Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations.
Iwai K, Minamisawa T, Suga K, Yajima Y, Shiba K.
Journal of Extracellular Vesicles 2016 May; 5:30829.
Application：WB, Human, Human saliva.
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All-trans retinoic acid suppresses malignant characteristics of CD133-positive thyroid cancer stem cells and induces apoptosis.
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