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Last updated: 2017/7/16
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ENG monoclonal antibody, clone 3A9

  • Catalog # : MAB10524
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant ENG.
  • Immunogen:
  • Recombinant protein corresponding to human ENG.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 71
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) against HEK293 (1) and CD105-hIgGFc transfected HEK293 (2) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues (A) and stomach cancer tissues (B) using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • Immunofluorescence analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green) and negative control (purple).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2022
  • Gene Name:
  • ENG
  • Gene Alias:
  • CD105,END,FLJ41744,HHT1,ORW,ORW1
  • Gene Description:
  • endoglin
  • Gene Summary:
  • This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • CD105 antigen,OTTHUMP00000022221,Osler-Rendu-Weber syndrome 1
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