ENG monoclonal antibody, clone 3A9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) against HEK293 (1) and CD105-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues (A) and stomach cancer tissues (B) using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
Immunofluorescence analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ENG.
Immunogen
Recombinant protein corresponding to human ENG.
Host
Mouse
Theoretical MW (kDa)
71
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) against HEK293 (1) and CD105-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues (A) and stomach cancer tissues (B) using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Immunofluorescence analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Flow Cytometry
Flow cytometric analysis of HepG2 cells using ENG monoclonal antibody, clone 3A9 (Cat # MAB10524) (green) and negative control (purple). -
Gene Info — ENG
Entrez GeneID
2022Gene Name
ENG
Gene Alias
CD105, END, FLJ41744, HHT1, ORW, ORW1
Gene Description
endoglin
Gene Ontology
HyperlinkGene Summary
This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD105 antigen|OTTHUMP00000022221|Osler-Rendu-Weber syndrome 1
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Interactome
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Disease
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