Western blot analysis using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) against HeLa (1) and PC-12 (2) cell lysate.
Immunofluorescence analysis of HeLa cells using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HeLa cells using CCNB1 monoclonal antibody, clone 5G6 (Cat # MAB10520) (green) and negative control (purple).
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq