CASP8 monoclonal antibody, clone 1H11
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519) against HeLa (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), COS-7 (5) and PC-12 (6) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human liver cancer (B) tissues using CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519).
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519) (blue) and negative control (red).
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CASP8.
Immunogen
Recombinant protein corresponding to human CASP8.
Host
Mouse
Theoretical MW (kDa)
26
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis using CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519) against HeLa (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), COS-7 (5) and PC-12 (6) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human liver cancer (B) tissues using CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA detection with CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519).Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using CASP8 monoclonal antibody, clone 1H11 (Cat # MAB10519) (blue) and negative control (red). -
Gene Info — CASP8
Entrez GeneID
841Gene Name
CASP8
Gene Alias
ALPS2B, CAP4, FLICE, FLJ17672, MACH, MCH5, MGC78473
Gene Description
caspase 8, apoptosis-related cysteine peptidase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq
Other Designations
FADD-homologous ICE/CED-3-like protease|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|Mch5 isoform alpha|OTTHUMP00000163718|OTTHUMP00000163719|OTTHUMP00000163720|caspase 8|caspase 8, apoptosis-related cysteine protease|cysteine protease|procaspase-8
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com