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Last updated: 2017/2/26
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CARM1 monoclonal antibody, clone 3H2

  • Catalog # : MAB10518
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CARM1.
  • Immunogen:
  • Recombinant protein corresponding to human CARM1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 65
  • Reactivity:
  • Human, Monkey, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) against MCF-7 (1), HeLa (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), COS-7 (8), and PC-12 (9) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and ovarian cancer tissues (B) using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of LoVo cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • CARM1
  • Gene Alias:
  • PRMT4
  • Gene Description:
  • coactivator-associated arginine methyltransferase 1
  • Gene Summary:
  • Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM
  • Other Designations:
  • coactivator-associated arginine methyltransferase-1,protein arginine N-methyltransferase 4
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