CARM1 monoclonal antibody, clone 3H2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) against MCF-7 (1), HeLa (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), COS-7 (8), and PC-12 (9) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and ovarian cancer tissues (B) using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of LoVo cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CARM1.
Immunogen
Recombinant protein corresponding to human CARM1.
Host
Mouse
Theoretical MW (kDa)
65
Reactivity
Human, Monkey, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) against MCF-7 (1), HeLa (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), COS-7 (8), and PC-12 (9) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and ovarian cancer tissues (B) using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of LoVo cells using CARM1 monoclonal antibody, clone 3H2 (Cat # MAB10518) (green) and negative control (purple). -
Gene Info — CARM1
Entrez GeneID
10498Gene Name
CARM1
Gene Alias
PRMT4
Gene Description
coactivator-associated arginine methyltransferase 1
Omim ID
603934Gene Ontology
HyperlinkGene Summary
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 [PubMed 11724789]).[supplied by OMIM
Other Designations
coactivator-associated arginine methyltransferase-1|protein arginine N-methyltransferase 4
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Interactome
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Disease
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