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Last updated: 2017/8/13
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CA9 monoclonal antibody, clone 2D3

  • Catalog # : MAB10517
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CA9.
  • Immunogen:
  • Recombinant protein corresponding to human CA9.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 50
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) against HeLa (1) and A-549 (2) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human stomach tissues (A) and colon tissues (B) using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) with DAB staining.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of NTERA-2 cells using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 768
  • Gene Name:
  • CA9
  • Gene Alias:
  • CAIX,MN
  • Gene Description:
  • carbonic anhydrase IX
  • Gene Summary:
  • Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022773,RCC-associated protein G250,carbonic dehydratase
  • Gene Pathway
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