CA9 monoclonal antibody, clone 2D3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) against HeLa (1) and A-549 (2) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human stomach tissues (A) and colon tissues (B) using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) with DAB staining.
Flow Cytometry
Flow cytometric analysis of NTERA-2 cells using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CA9.
Immunogen
Recombinant protein corresponding to human CA9.
Host
Mouse
Theoretical MW (kDa)
50
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) against HeLa (1) and A-549 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human stomach tissues (A) and colon tissues (B) using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of NTERA-2 cells using CA9 monoclonal antibody, clone 2D3 (Cat # MAB10517) (green) and negative control (purple). -
Gene Info — CA9
Entrez GeneID
768Gene Name
CA9
Gene Alias
CAIX, MN
Gene Description
carbonic anhydrase IX
Omim ID
603179Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq
Other Designations
OTTHUMP00000022773|RCC-associated protein G250|carbonic dehydratase
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Interactome
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Pathway
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Disease
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