REL monoclonal antibody, clone 1E7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using REL monoclonal antibody, clone 1E7 (Cat # MAB10516) against Jurkat (1), NIH/3T3 (2), HeLa (3), HEK293 (4) and Raji (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues (A) and liver cancer tissues (B) using REL monoclonal antibody, clone 1E7 (Cat # MAB10516) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of U251 cells using REL monoclonal antibody, clone 1E7 (Cat # MAB10516) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Enzyme-linked Immunoabsorbent Assay
ELISA detection with REL monoclonal antibody, clone 1E7 (Cat # MAB10516).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant REL.
Immunogen
Recombinant protein corresponding to human REL.
Host
Mouse
Theoretical MW (kDa)
78
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using REL monoclonal antibody, clone 1E7 (Cat # MAB10516) against Jurkat (1), NIH/3T3 (2), HeLa (3), HEK293 (4) and Raji (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues (A) and liver cancer tissues (B) using REL monoclonal antibody, clone 1E7 (Cat # MAB10516) with DAB staining.Immunofluorescence
Immunofluorescence analysis of U251 cells using REL monoclonal antibody, clone 1E7 (Cat # MAB10516) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
ELISA detection with REL monoclonal antibody, clone 1E7 (Cat # MAB10516). -
Gene Info — REL
Entrez GeneID
5966Gene Name
REL
Gene Alias
C-Rel
Gene Description
v-rel reticuloendotheliosis viral oncogene homolog (avian)
Omim ID
164910Gene Ontology
HyperlinkGene Summary
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM
Other Designations
C-Rel proto-oncogene protein|oncogene REL, avian reticuloendotheliosis|v-rel avian reticuloendotheliosis viral oncogene homolog|v-rel reticuloendotheliosis viral oncogene homolog
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Interactome
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Disease
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