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Last updated: 2017/6/25
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ACTB monoclonal antibody, clone 8H10D10

  • Catalog # : MAB10501
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of ACTB.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to human ACTB.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 42
  • Reactivity:
  • Hamster, Human, Monkey, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG2b
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC-12 (5), HEK293 (6), COS (7), A-549 (8) and MCF-7 (9) cell lysate.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of SK-BR-3 (upper) and A-549 (bottom) cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of MCF-7 cells using ACTB monoclonal antibody, clone 8H10D10 (Cat # MAB10501) (A) and negative control (B).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 60
  • Gene Name:
  • ACTB
  • Gene Alias:
  • PS1TP5BP1
  • Gene Description:
  • actin, beta
  • Gene Summary:
  • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
  • Other Designations:
  • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
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