PPM1A monoclonal antibody, clone p6c7
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PPM1A.
Immunogen
Recombinant protein corresponding to amino acids 1-382 of human PPM1A.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G affinity chromatography
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane 1: mouse kidney tissue lysate; Lane 2: mouse brain tissue lysate; Lane 3: mouse liver tissue lysate.Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate; Lane 2: HeLa cell lysate; Lane 3: K562 cell lysate; Lane 4: MCF7 cell lysate; Lane 5: A549 cell lysate; Lane 6: Raji cell lysate. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL.Immunocytochemistry
Immunocytochemistry analysis of HeLa cells. The cell was stained with PPM1A monoclonal antibody, clone p6c7 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — PPM1A
Entrez GeneID
5494GeneBank Accession#
NM_177952Protein Accession#
P35813Gene Name
PPM1A
Gene Alias
FLJ42306, MGC9201, PP2C-ALPHA, PP2CA
Gene Description
protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
Omim ID
606108Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
protein phosphatase 1A|protein phosphatase 2C alpha isoform
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone morphogenetic protein signaling pathway.
Duan X, Liang YY, Feng XH, Lin X.
The Journal of Biological Chemistry 2006 Dec; 281(48):36526.
Application:WB, Mouse, Mv1Lu cells.
-
PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling.
Lin X, Duan X, Liang YY, Su Y, Wrighton KH, Long J, Hu M, Davis CM, Wang J, Brunicardi FC, Shi Y, Chen YG, Meng A, Feng XH.
Cell 2006 Jun; 125(5):915.
Application:IF, IP, WB, Human, Mink, HaCaT, HEK 293T, Mv1Lu cells.
-
Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone morphogenetic protein signaling pathway.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com