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Last updated: 2017/1/22
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APOE monoclonal antibody, clone 1H4

  • Catalog # : MAB10490
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant APOE.
  • Immunogen:
  • Recombinant protein corresponding to human APOE.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 36
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis using APOE monoclonal antibody, clone 1H4 (Cat # MAB10490) against HEK293 (1) and ApoE (aa : 20-267)-hIgGFc transfected HEK293 (2) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (A) and brain tissues (B) using APOE monoclonal antibody, clone 1H4 (Cat # MAB10490) with DAB staining.
  • ELISA
  • ELISA
  • ELISA detection with APOE monoclonal antibody, clone 1H4 (Cat # MAB10490).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HepG2 cells using APOE monoclonal antibody, clone 1H4 (Cat # MAB10490) (green) and negative control (purple).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 348
  • Gene Name:
  • APOE
  • Gene Alias:
  • AD2,LPG,MGC1571,apoprotein
  • Gene Description:
  • apolipoprotein E
  • Gene Summary:
  • Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq
  • Other Designations:
  • apolipoprotein E3
  • Gene Pathway
  • Related Disease
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