ALPP monoclonal antibody, clone 3E5
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant ALPP.
Immunogen
Recombinant protein corresponding to human ALPP.
Host
Mouse
Theoretical MW (kDa)
58
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using ALPP monoclonal antibody, clone 3E5 (Cat # MAB10486) against HepG2 (1), A-431 (2) and MCF-7 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A), stomach cancer (B) using ALPP monoclonal antibody, clone 3E5 (Cat # MAB10486) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ALPP
Entrez GeneID
250Gene Name
ALPP
Gene Alias
ALP, FLJ61142, PALP, PLAP
Gene Description
alkaline phosphatase, placental (Regan isozyme)
Omim ID
171800Gene Ontology
HyperlinkGene Summary
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq
Other Designations
alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase
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Interactome
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