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Last updated: 2017/7/23
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ACTA2 monoclonal antibody, clone 4A4

  • Catalog # : MAB10482
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of ACTA2.
  • Immunogen:
  • A synthetic peptide corresponding to human ACTA2.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 42
  • Reactivity:
  • Human, Monkey, Mouse, Rat
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) against HeLa (1), A-431 (2), Jurkat (3), K-562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human duodenum tissues (A) and human esophagus tissues (B) using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HepG2 cells using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • ELISA
  • ELISA detection with ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using ACTA2 monoclonal antibody, clone 4A4 (Cat # MAB10482) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 59
  • Gene Name:
  • ACTA2
  • Gene Alias:
  • AAT6,ACTSA
  • Gene Description:
  • actin, alpha 2, smooth muscle, aorta
  • Gene Summary:
  • The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020042,alpha 2 actin,alpha-cardiac actin,growth-inhibiting gene 46
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