CALR monoclonal antibody, clone 1G6A7
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) against HeLa (1), A-549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.
Western Blot (Transfected lysate)
Western blot analysis using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CALR cDNA (2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissue using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459).
Immunofluorescence
Confocal immunofluorescence analysis of SK-BR-3 (A) and A-549 (B) cells using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of 3T3-L1 cells using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) (green). Blue: DRAQ5 fluorescent DNA dye.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide CALR.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human Calreticulin.
Sequence
EEEDVPGQAKDELC
Host
Mouse
Theoretical MW (kDa)
48
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) against HeLa (1), A-549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.Western Blot (Transfected lysate)
Western blot analysis using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CALR cDNA (2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissue using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459).Immunofluorescence
Confocal immunofluorescence analysis of SK-BR-3 (A) and A-549 (B) cells using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) (green).
Red: Actin filaments have been labeled with DY-554 phalloidin.
Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal immunofluorescence analysis of 3T3-L1 cells using CALR monoclonal antibody, clone 1G6A7 (Cat # MAB10459) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CALR
Entrez GeneID
811Gene Name
CALR
Gene Alias
CRT, FLJ26680, RO, SSA, cC1qR
Gene Description
calreticulin
Omim ID
109091Gene Ontology
HyperlinkGene Summary
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq
Other Designations
Sicca syndrome antigen A (autoantigen Ro; calreticulin)|autoantigen Ro
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Interactome
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Disease
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