CANX monoclonal antibody, clone 3H4A7
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide CANX.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human Calnexin.
Sequence
CEAAEERPWLWVVYILTVAL
Host
Mouse
Theoretical MW (kDa)
90
Reactivity
Human
Form
Liquid
Isotype
IgG2b
Recommend Usage
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascitic (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CANX monoclonal antibody, clone 3H4A7 (Cat # MAB10458) against A-431 (1), HeLa (2), MCF-7 (3) and A-549 (4) cell lysate.Immunofluorescence
Confocal immunofluorescence analysis of HeLa cells using CANX monoclonal antibody, clone 3H4A7 (Cat # MAB10458) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CANX
Entrez GeneID
821Gene Name
CANX
Gene Alias
CNX, FLJ26570, IP90, P90
Gene Description
calnexin
Omim ID
114217Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq
Other Designations
major histocompatibility complex class I antigen-binding protein p88
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