ASS1 monoclonal antibody, clone 2B10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) against A-431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A-549 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) against human ASS1 (aa 40-236) recombinant protein. (Expected MW is 47 kDa)
Immunofluorescence
Immunofluorescence analysis of HeLa cells using ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) (green). Blue: DRAQ5 fluorescent DNA dye.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448).
Flow Cytometry
Flow cytometric analysis of Jurkat cells using ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant ASS1.
Immunogen
Recombinant protein corresponding to human ASS1.
Host
Mouse
Theoretical MW (kDa)
47
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) against A-431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A-549 (6), NIH/3T3 (7), PC-12 (8) and COS-7 (9) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) against human ASS1 (aa 40-236) recombinant protein. (Expected MW is 47 kDa)Immunofluorescence
Immunofluorescence analysis of HeLa cells using ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448).Flow Cytometry
Flow cytometric analysis of Jurkat cells using ASS1 monoclonal antibody, clone 2B10 (Cat # MAB10448) (green) and negative control (red). -
Gene Info — ASS1
Entrez GeneID
445Gene Name
ASS1
Gene Alias
ASS, CTLN1
Gene Description
argininosuccinate synthetase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000022362|OTTHUMP00000022363|OTTHUMP00000022364|citrulline--aspartate ligase
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Interactome
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Pathway
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Disease
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