RAB25 monoclonal antibody, clone 3F12F3
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More Files
- More Functions
Western Blot
Western blot analysis using RAB25 monoclonal antibody, clone 3F12F3 (Cat # MAB10441) against truncated RAB25 recombinant protein (1), human overy carcinoma(2), stomach carcinoma (3), breast carcinoma (4) tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (A), breast carcinoma (B), esophagus carcinoma (C), skin carcinoma (D) tissue, showing cytoplasmic localization using RAB25 monoclonal antibody, clone 3F12F3 (Cat # MAB10441) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant RAB25.
Immunogen
Recombinant protein corresponding to human RAB25.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using RAB25 monoclonal antibody, clone 3F12F3 (Cat # MAB10441) against truncated RAB25 recombinant protein (1), human overy carcinoma(2), stomach carcinoma (3), breast carcinoma (4) tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (A), breast carcinoma (B), esophagus carcinoma (C), skin carcinoma (D) tissue, showing cytoplasmic localization using RAB25 monoclonal antibody, clone 3F12F3 (Cat # MAB10441) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAB25
Entrez GeneID
57111Gene Name
RAB25
Gene Alias
CATX-8
Gene Description
RAB25, member RAS oncogene family
Gene Ontology
HyperlinkGene Summary
RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associated moieties to the cell surface (Caswell et al., 2007 [PubMed 17925226]).[supplied by OMIM
Other Designations
OTTHUMP00000018847
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Interactome
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Publication Reference
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High RAB25 expression is associated with good clinical outcome in patients with locally advanced head and neck squamous cell carcinoma.
Téllez-Gabriel M, Arroyo-Solera I, León X, Gallardo A, López M, Céspedes MV, Casanova I, López-Pousa A, Quer M, Mangues MA, Barnadas A,Mangues R, Pavón MA.
Cancer Medicine 2013 Dec; 2(6):950.
Application：IHC-P, Human, Head and neck squamous cell carcinoma.
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High RAB25 expression is associated with good clinical outcome in patients with locally advanced head and neck squamous cell carcinoma.
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