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BRAF monoclonal antibody, clone 1H12

  • Catalog # : MAB10422
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant BRAF.
  • Immunogen:
  • Recombinant protein corresponding to human BRAF.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 87
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    ELISA (1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascitic (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) against HeLa (1), HL-60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human testis tissue using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422).
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of MCF-7 (A) and HepG2 (B) cells using BRAF monoclonal antibody, clone 1H12 (Cat # MAB10422) (green).
    Red: Actin filaments have been labeled with DY-554 phalloidin.
    Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 673
  • Gene Name:
  • BRAF
  • Gene Alias:
  • B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
  • Gene Description:
  • v-raf murine sarcoma viral oncogene homolog B1
  • Gene Summary:
  • This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
  • Other Designations:
  • 94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1
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