VIM monoclonal antibody, clone 4F2E9
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of VIM monoclonal antibody, clone 4F2E9 (Cat # MAB10419) against HeLa (1), COS (2), HEK293 (3) and U20S (4) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using VIM monoclonal antibody, clone 4F2E9 (Cat # MAB10419)
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant VIM.
Immunogen
Recombinant protein corresponding to amino acids 2-466 of human VIM.
Host
Mouse
Theoretical MW (kDa)
54
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of VIM monoclonal antibody, clone 4F2E9 (Cat # MAB10419) against HeLa (1), COS (2), HEK293 (3) and U20S (4) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using VIM monoclonal antibody, clone 4F2E9 (Cat # MAB10419)Enzyme-linked Immunoabsorbent Assay
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Gene Info — VIM
Entrez GeneID
7431Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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