RAB25 monoclonal antibody, clone 3F12
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human esophagus tissue (A) and human lung cancer (B) using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of A-549 cells using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant RAB25.
Immunogen
Recombinant protein corresponding to human RAB25.
Host
Mouse
Theoretical MW (kDa)
24
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human esophagus tissue (A) and human lung cancer (B) using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) with DAB staining.Immunofluorescence
Immunofluorescence analysis of A-549 cells using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) (green) and negative control (purple). -
Gene Info — RAB25
Entrez GeneID
57111Gene Name
RAB25
Gene Alias
CATX-8
Gene Description
RAB25, member RAS oncogene family
Gene Ontology
HyperlinkGene Summary
RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associated moieties to the cell surface (Caswell et al., 2007 [PubMed 17925226]).[supplied by OMIM
Other Designations
OTTHUMP00000018847
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