Mouse monoclonal antibody raised against recombinant RAB25.
Recombinant protein corresponding to human RAB25.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human esophagus tissue (A) and human lung cancer (B) using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) with DAB staining.
Immunofluorescence analysis of A-549 cells using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH/3T3 cells using RAB25 monoclonal antibody, clone 3F12 (Cat # MAB10406) (green) and negative control (purple).
RAB proteins, such as RAB25, are members of the RAS superfamily of small GTPases that are involved in membrane trafficking. Members of the RAB11 subfamily, including RAB25, control the return of internalized membrane-associated moieties to the cell surface (Caswell et al., 2007 [PubMed 17925226]).[supplied by OMIM