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Last updated: 2017/10/15
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PTEN monoclonal antibody, clone 1B8

  • Catalog # : MAB10405
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant PTEN.
  • Immunogen:
  • Recombinant protein corresponding to human PTEN.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 54
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) against HeLa (1) and NIH/3T3 (2) cell lysate.
  • Immunofluorescence
  • Immunofluorescence
  • Confocal immunofluorescence analysis of HeLa (A) and HepG2 (B) cells using PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) (green).
    Red: Actin filaments have been labeled with DY-554 phalloidin.
    Blue: DRAQ5 fluorescent DNA dye.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using PTEN monoclonal antibody, clone 1B8 (Cat # MAB10405) (B) and negative control (A).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5728
  • Gene Name:
  • PTEN
  • Gene Alias:
  • 10q23del,BZS,MGC11227,MHAM,MMAC1,PTEN1,TEP1
  • Gene Description:
  • phosphatase and tensin homolog
  • Gene Summary:
  • This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq
  • Other Designations:
  • MMAC1 phosphatase and tensin homolog deleted on chromosome 10,OTTHUMP00000020032,mutated in multiple advanced cancers 1
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