MAPKAPK5 monoclonal antibody, clone 7H10B4
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant MAPKAPK5.
Immunogen
Recombinant protein corresponding to human MAPKAPK5.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis using MAPKAPK5 monoclonal antibody, clone 7H10B4 (Cat # MAB10404) against truncated MAPKAPK5 recombinant protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver carcinoma (A), esophagus carcinoma (B), normal spleen tissue(C), breast carcinoma (D), showing nuclear and cytoplasmic localization using MAPKAPK5 monoclonal antibody, clone 7H10B4 (Cat # MAB10404) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAPKAPK5
Entrez GeneID
8550Gene Name
MAPKAPK5
Gene Alias
PRAK
Gene Description
mitogen-activated protein kinase-activated protein kinase 5
Omim ID
606723Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
p38-regulated/activated protein kinase
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Interactome
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