STAT3 monoclonal antibody, clone 3B5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) against HeLa (1),NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS-7 (5) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue (A) and lung cancer tissue (B) using STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HeLa cells using STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant STAT3.
Immunogen
Recombinant protein corresponding to human STAT3.
Host
Mouse
Theoretical MW (kDa)
88
Reactivity
Human, Monkey, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) against HeLa (1),NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS-7 (5) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human mammary cancer tissue (A) and lung cancer tissue (B) using STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using STAT3 monoclonal antobody, clone 3B5 (Cat # MAB10397) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STAT3
Entrez GeneID
6774Gene Name
STAT3
Gene Alias
APRF, FLJ20882, HIES, MGC16063
Gene Description
signal transducer and activator of transcription 3 (acute-phase response factor)
Omim ID
102582Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
DNA-binding protein APRF|acute-phase response factor|signal transducer and activator of transcription 3
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