SRC monoclonal antibody, clone 1F11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against MCF-7 (1), A-431 (2), HeLa (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and COS-7 (7) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against human SRC (aa 1-189) recombinant protein. (Expected MW is 47.8 kDa).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder cancer (A) and human esophageal (B) tissue using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) with DAB staining.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SRC.
Immunogen
Recombinant protein corresponding to human SRC.
Host
Mouse
Theoretical MW (kDa)
60
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against MCF-7 (1), A-431 (2), HeLa (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and COS-7 (7) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) against human SRC (aa 1-189) recombinant protein. (Expected MW is 47.8 kDa).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human bladder cancer (A) and human esophageal (B) tissue using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) with DAB staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using SRC monoclonal antobody, clone 1F11 (Cat # MAB10395) (green) and negative control (red). -
Gene Info — SRC
Entrez GeneID
6714Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Interactome
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Pathway
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Disease
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