SMC1A monoclonal antibody, clone 5B6
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393) against K-562 (1), Jurkat (2) and A-549 (3) cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon using SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393).
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HeLa cells using SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SMC1A.
Immunogen
Recombinant protein corresponding to human SMC1A.
Host
Mouse
Theoretical MW (kDa)
143
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393) against K-562 (1), Jurkat (2) and A-549 (3) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon using SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393).Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393) (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using SMC1A monoclonal antobody, clone 5B6 (Cat # MAB10393) (green) and negative control (purple). -
Gene Info — SMC1A
Entrez GeneID
8243Gene Name
SMC1A
Gene Alias
CDLS2, DKFZp686L19178, DXS423E, KIAA0178, MGC138332, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB
Gene Description
structural maintenance of chromosomes 1A
Gene Ontology
HyperlinkGene Summary
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq
Other Designations
OTTHUMP00000061876|SMC1 (structural maintenance of chromosomes 1, yeast)-like 1|SMC1 structural maintenance of chromosomes 1-like 1|segregation of mitotic chromosomes 1
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Interactome
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Pathway
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Disease
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