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Last updated: 2017/8/20
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SMAD4 monoclonal antibody, clone 4G1C6

  • Catalog # : MAB10392
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant SMAD4.
  • Immunogen:
  • Recombinant protein corresponding to human SMAD4.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 65
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392) against A-431 (1), SK-N-SH (2), K-562 (3), HepG2 (4) and HUVE12 (5) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392)
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of NIH/3T3 cells using SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of K-562 cells using SMAD4 monoclonal antobody, clone 4G1C6 (Cat # MAB10392) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4089
  • Gene Name:
  • SMAD4
  • Gene Alias:
  • DPC4,JIP,MADH4
  • Gene Description:
  • SMAD family member 4
  • Other Designations:
  • MAD, mothers against decapentaplegic homolog 4,OTTHUMP00000163548,SMAD, mothers against DPP homolog 4,deleted in pancreatic carcinoma locus 4,mothers against decapentaplegic homolog 4,mothers against decapentaplegic, Drosophila, homolog of, 4
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