RBP4 monoclonal antibody, clone 4C2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant RBP4.
Immunogen
Recombinant protein corresponding to human RBP4.
Host
Mouse
Theoretical MW (kDa)
23
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using RBP4 monoclonal antobody, clone 4C2 (Cat # MAB10385) against HEK293 (1) and RBP4-hIgGFc transfected HEK293 (2) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue (A) and stomach cancer tissue (B) using RBP4 monoclonal antobody, clone 4C2 (Cat # MAB10385) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HepG2 cells using RBP4 monoclonal antobody, clone 4C2 (Cat # MAB10385) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells using RBP4 monoclonal antobody, clone 4C2 (Cat # MAB10385) (green) and negative control (purple). -
Gene Info — RBP4
Entrez GeneID
5950Gene Name
RBP4
Gene Alias
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Gene Description
retinol binding protein 4, plasma
Omim ID
180250Gene Ontology
HyperlinkGene Summary
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq
Other Designations
OTTHUMP00000020114|OTTHUMP00000020116|retinol-binding protein 4, interstitial|retinol-binding protein 4, plasma
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Interactome
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Disease
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