PDK1 monoclonal antibody, clone 4A11
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PDK1.
Immunogen
Recombinant protein corresponding to human PDK1.
Host
Mouse
Theoretical MW (kDa)
44
Reactivity
Human, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using PDK1 monoclonal antobody, clone 4A11 (Cat # MAB10380) against NIH/3T3 (1), HeLa (2), Jurkat (3), HepG2 (4), PC-12 (5), and COS-7 (6) cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (A) and brain tissue (B) using PDK1 monoclonal antobody, clone 4A11 (Cat # MAB10380) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HeLa cells using PDK1 monoclonal antobody, clone 4A11 (Cat # MAB10380) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of LoVo cells using PDK1 monoclonal antobody, clone 4A11 (Cat # MAB10380) (green) and negative control (purple). -
Gene Info — PDK1
Entrez GeneID
5163Gene Name
PDK1
Gene Alias
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Gene Description
pyruvate dehydrogenase kinase, isozyme 1
Omim ID
602524Gene Ontology
HyperlinkGene Summary
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq
Other Designations
mitochondrial pyruvate dehydrogenase kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1
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Interactome
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Pathway
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Disease
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