KARS monoclonal antibody, clone 8G12C1
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More Files
- More Functions
Western Blot
Western blot analysis using KARS monoclonal antibody, clone 8G12C1 (Cat # MAB10375) against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa 90-174) and full length KARS (aa 1-188) transfected CHO-K1 cell lysate (3).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using KARS monoclonal antibody, clone 8G12C1 (Cat # MAB10375) with DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using KARS monoclonal antibody, clone 8G12C1 (Cat # MAB10375) with DAB staining.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KARS.
Immunogen
Recombinant protein corresponding to amino acids 90-174 of human KARS.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis using KARS monoclonal antibody, clone 8G12C1 (Cat # MAB10375) against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa 90-174) and full length KARS (aa 1-188) transfected CHO-K1 cell lysate (3).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using KARS monoclonal antibody, clone 8G12C1 (Cat # MAB10375) with DAB staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using KARS monoclonal antibody, clone 8G12C1 (Cat # MAB10375) with DAB staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KARS
Entrez GeneID
3735Gene Name
KARS
Gene Alias
KARS2, KIAA0070, KRS
Gene Description
lysyl-tRNA synthetase
Omim ID
601421Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
lysine tRNA ligase
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Interactome
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Pathway
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Disease
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